Part:BBa_J45250

Stationary-phase-dependent banana odor generator

The stationary phase dependent banana odor generator (BBa_J45250) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_J45992) and an odor enzyme generator (BBa_J45199). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

Usage and Biology

• Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
• See BBa_J45014 for details.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 327
  Illegal BamHI site found at 1654
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 754
  Illegal SapI.rc site found at 1762